holographic
technology
overview

fast
versatile
precise

Real-time analysis – record data up to 22 frames per second

1000 times larger depth of focus compared to conventional microscopes

In-situ recording – no sample collection, no staining, no filtering

Capable of imaging objects ranging in size from microns to millimeters in the same volume

Quantitative Phase Imaging allows for precise determination of the objects’ refractive index

Technology overview of 4-Deep's Submersible Microscope

submersible
microscope

The design of 4Deep’s microscope shows the location of the Point Source (laser), Sample Space, Camera, as well as the cone of light (green) emitted by the Point Source

Objects that are located within the light cone will be imaged by microscope’s camera

Submersible microscope contains no pumps, plumbing, lens or moving parts

submersible
microscope

The design of 4Deep’s microscope shows the location of the Point Source (laser), Sample Space, Camera, as well as the cone of light (green) emitted by the Point Source

Objects that are located within the light cone will be imaged by microscope’s camera

Submersible microscope contains no pumps, plumbing, lens or moving parts

Technology overview of 4-Deep's Submersible Microscope

capture sample volumes

One 2D hologram contains information about the entire 3D space

Image 30 -100 μl per hologram in standard instruments, customizable to several ml

Micron range resolution throughout the volume

Submersible microscope can image clearly at fluid speeds up to 4 m/s

3D Scanning Sample Volumes

4D – tracking

Open space design – no flow cells

Allows unrestricted tracking of objects in their natural environment

Calculate precise particle positions and velocities

4D – tracking

Open space design – no flow cells
Allows unrestricted tracking of objects in their natural environment
Calculate precise particle velocities

digital refocusing

Capture holograms rapidly without worrying about focusing

Digitally select 2D image planes after hologram is recorded

Slice through sample volume wherever needed

Autofocus automatically finds objects in perfect focus