holographic
fast
versatile
precise
Real-time analysis – record data up to 22 frames per second
1000 times larger depth of focus compared to conventional microscopes
In-situ recording – no sample collection, no staining, no filtering
Capable of imaging objects ranging in size from microns to millimeters in the same volume
Quantitative Phase Imaging allows for precise determination of the objects’ refractive index
submersible
microscope
The design of 4Deep’s microscope shows the location of the Point Source (laser), Sample Space, Camera, as well as the cone of light (green) emitted by the Point Source
Objects that are located within the light cone will be imaged by microscope’s camera
Submersible microscope contains no pumps, plumbing, lens or moving parts
submersible
microscope
The design of 4Deep’s microscope shows the location of the Point Source (laser), Sample Space, Camera, as well as the cone of light (green) emitted by the Point Source
Objects that are located within the light cone will be imaged by microscope’s camera
Submersible microscope contains no pumps, plumbing, lens or moving parts
capture sample volumes
One 2D hologram contains information about the entire 3D space
Image 30 -100 μl per hologram in standard instruments, customizable to several ml
Micron range resolution throughout the volume
Submersible microscope can image clearly at fluid speeds up to 4 m/s
4D – tracking
Open space design – no flow cells
Allows unrestricted tracking of objects in their natural environment
Calculate precise particle positions and velocities
4D – tracking
Open space design – no flow cells
Allows unrestricted tracking of objects in their natural environment
Calculate precise particle velocities
digital refocusing
Capture holograms rapidly without worrying about focusing
Digitally select 2D image planes after hologram is recorded
Slice through sample volume wherever needed
Autofocus automatically finds objects in perfect focus