4 Deep | Submersible Underwater Microscopy
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Microscopy for the bigger picture.

Inwater Holographic Microscope Systems.

Real-time, in-situ observation of particles and micro-organisms in all aquatic environments.

Image courtesy of NASA

NEW! Technology Fast 50 by Deloitte awarded to 4Deep

Deloitte logo

4Deep inwater imaging is honored to be the only Atlantic Canadian company recognized with a Companies-to-Watch Award, as part of the Deloitte 17th annual Technology Fast 50TM Awards for its innovative technology, management expertise and early stage growth.

NEW! Quantitative Phase Imaging

The capability of measuring thickness of an object to sub-micron accuracy is now a part of Octopus 1.5. Click here to read more about Quantitative Phase Imaging

NEW! Submersible Microscopes now have Pulsed Lasers.

Light pulse duration as fast as 1 microsecond.
Enables clear images with 1 micron resolution in fast flowing water conditions.
Submersible tested at towing speeds of up to 4 Knots.

System Components

Submersible Microscope

Submersible Microscope

  • Real time imaging
  • NEW high speed pulsed laser
  • Large imaging volume
  • Full depth of field through volume
  • Rapid image acquisition
  • Tested at towing speeds of 4 knots
  • Simple, rugged design
  • No moving parts
  • Small and lightweight
  • Deploy from buoy, ROV, AUV, vessel
  • Deploy to 6,000 m depth (custom models)
Desktop Microscope

Desktop Microscope

  • In-situ observation
  • Automatic cell counting
  • No staining or prep
  • 0.5 µm resolution
  • Variable numerical aperture
  • X–Y–Z translation stage
Cuvette Microscope

Cuvette Microscope

  • Instantaneous liquid sampling
  • Small, lightweight and portable
  • Standard cuvettes 12 x 12 x 45 mm
  • Compatible with all software
  • 2 micron resolution
  • Biomedical, environmental, industrial applications
  • NEW Teachers Workbook of lab ideas
Patented Software

Patented Software

  • Collect / reconstruct full size holograms at 16 frames per second.
  • 4D analysis of holograms in volume and time
  • Open data formats
  • NEW Quantitative Phase Imaging
  • Particles and microorganisms can be detected, sized, counted and characterized automatically.

∞ better technology

The most powerful microscopy system available.


lag time real-time data


times more spatial resolution


times faster image acquisition


times faster hologram reconstruction

Holographic Microscopy



Our microscopes use a laser (A) and camera (B) to image, count, size and characterize objects in any body of water within the sample area (C).   Resolution extends to objects as small as 0.5 µm and as large as 3 mm.   Our software generates live 3D & 4D holograms (D).
4Deep Microscopy

4Deep Microscopy

Conventional Microscopy

Conventional Microscopy

Narrow focal plane. Staining and prep required. Constrains objects to plane.
Holographic Microscopy

Holographic Microscopy

Everything in field of view is in focus. No staining or prep. Capture movement of objects in 3D.